There are 24 clubs in League One. Each club plays every other club twice (once at home and once away). Three points are awarded for a win, one for a draw and zero for a loss. At the end of the season a table of the final League standings is determined, based on the following criteria in this order: points obtained, goal difference, goals scored, an aggregate of the results between two or more clubs (ranked using the previous three criteria) and, finally, a series of one or more play-off matches.

At the end of each season the top two clubs, together with the winner of the play-offs between the clubs which finished in 3rd–6th position, are promoted to EFL Championship and are replaced by the three clubs that finished at the bottom of that division.

Similarly, the four clubs that finished at the bottom of EFL League One are relegated to EFL League Two and are replaced by the top three clubs and the club that won the 4th–7th place play-offs in that division.